The template strand is the strand which serves as the template for the mRNA synthesis during transcription. That means, the template strand is the DNA strand in the double-stranded DNA which is responsible for the amino acid sequence of the synthesized polynucleotide chain. The template strand is also called the antisense strand or the positive strand. The template strand consists of a sequence of anti-codons which are the nucleotide triplets found in the tRNA s individually.
The anti-codon is complementary to codons in the non-template or the coding strand. The synthesizing mRNA is temporally attached to the template strand by forming hydrogen bonds with the complementary nucleotides in the template strand. RNA polymerase adds uracil as the complementary nucleotide to the mRNA strand for adenine in the template strand instead of thymine.
The template strand in the transcription is shown in figure 1. The DNA strand which serves as the non-template strand during transcription is referred to as the coding strand. Hence, coding strand is incapable of serving as the template during transcription.
The coding strand contains codons, which are the nucleotide triplets which specify a unique amino acid in the polypeptide chain. These codons collectively make the genetic code, which is a universal feature in almost all the living forms on the earth.
Coding strand during the transcription is shown in figure 2. Hence, RNA polymerase is the one which decides the initiation of transcription and termination of the translation process. As we have discussed the mRNA will form complementary base sequences to that of the template strand. The base sequence of mRNA similar to the coding strand, will have the same nucleotide bases, except for thymine. In mRNA, uracil is the nitrogenous base that replaces thymine. As the RNAP uses the template strand to transcribe the mRNA, the other strand will be the sense strand that will form a complementary strand to that of the template.
It contains triplet codons , which code for the specific amino acid to build proteins through the mRNA translation. The coding strand will produce complementary pairs relative to the template strand according to the Watson and Crick model.
Your email address will not be published. Table of Contents Photoautotroph Definition The organisms that can make their energy in presence of light and carbon dioxide are known as photoautotrophs. The process of preparing their own food is called photosynthesis. The word. They are hollow tubes made of the proteins alpha and beta tubulins. Microtubulin form a network of protein filaments extended throughout the. It actually acts as a template for the synthesis of RNA.
In the molecule of RNA, uracil substitutes for Thymine. What is Gene? Definition, Structure, Expression, and Facts. Probing DNA polymerase-DNA interactions: examining the template strand in exonuclease complexes using 2-aminopurine fluorescence and acrylamide quenching.
Detection of template strand switching during initiation and termination of DNA replication of porcine circovirus. J Virol. DNA template-assisted inhibition of tyrosinase activity. Int J Biol Macromol. Share on facebook. Share on twitter.
Share on linkedin. Share on whatsapp. Share on email. Share on telegram. Share on pinterest.
0コメント